Walker missed two days with the ankle injury, which he sustained in the Packer's preseason opener vs. Kansas City Monday night.
An MRI confirmed that there was no break and Walker was able to return to the field Friday for the team's workout at Lambeau.
"He ran around pretty good," offensive coordinator Tom Rossley said.
During the team's Friday session, Walker was back in the starting lineup.
"I'm going to give it a go and see how it works out," Walker said of playing in tonight's game.
Injuries have had a big impact on the Packers' receiving corps. Starters Donald Driver (hamstring) and Robert Ferguson (tailbone) remain on the sidelined for this game, although Ferguson was able to return to practice Thursday. Before Walker's return, the scheduled starting tandem was Karsten Bailey and Carl Ford. Now Walker will line up with Bailey.